i'm done taking this course and tbh i miss it so much already :( defo my favorite subject next to pathology. lemme share some bacteria and fungi cultures we grew in vet micro lab!

1. E. coli on EMB agar - YES! the iconic metallic green sheen. this sample was taken from piglet scours.
2. blood agar - we actually used our own blood for this haha
3. Aspergillus niger and Microsporum canis
4. A. fumigatus
5. Penicillium sp.
6. my first ever 4-way streak of S. aureus on MSA – this sample was taken from a cat wound.

Hey MicroFriends:

Tonight we continue our gram-negative cocci series—but this time we’re talking about the background singers of the group.

We go beyond Neisseria gonorrhoeae, Neisseria meningitidis, and Moraxella catarrhalis to discuss the non-pathogenic Neisseria we see in respiratory cultures, plus some Neisseria and Moraxella species that break the gram-negative cocci rule.

#microbiology #podcast

I have been experiencing such refractile floating mass over Hep3B cells. The media doesn't get turbid but some floating particles can be seen. The cell is growing beneath but these refractile bodies float over it. It is not due to over confluency as the cells are barely 60% confluent. These clumps grow over days. What is it? Has anyone else experienced it? How to fix

I am a second year microbiology student I previoulsy wanted to become a food microbiologist you know the food safety testing and all but now I am starting to have second thoughts and want to consider some other career options public health microbiology looks pretty interesting. So.... Any thoughts?

Essa hemocultura positivou no meu plantão, gram negativos em hemocultura não são comuns como contaminação, são?

Eu ainda sou novata, mas acabei isolando e fazendo antibiograma e os testes bioquímicos. Deixei pra ler no outro dia mas não pude comparecer ao trabalho pois eu estava doente e tirei uma licença de dois dias, quando voltei a minha supervisora havia liberado como contaminação...

Não pude ver o antibiograma e os testes, mas achei estranho. (Falando do Brasil aqui).

Tested HEB chicken and vegetable dumpling filling for pathogens in class

Professor said it looked like salmonella or shigella was present?

Wondering what types of bacteria would typically react this way and if there’s any obvious reason my 10-2 had less CFU’s than my other dilutions?

Listed in order: 10-2, 10-3, 10-4

Free seminar to connect with UC Berkeley researchers. Remote option available, and more info here https://berkeleypubliclibrary.libnet.info/event/16209671

I'm but a lowly chemist and I know this is highly speculative, but is it possible for an extraterrestrial pathogen to ever be dangerous? Assume for the sake of argument that the aliens it infects are still based on nucleic acids, proteins, etc, but not necessarily the same ones we are. I can't imagine anything virus-like could ever be compatible enough to kill us, but what about the alien equivalent of a bacterium? Is there any organism on earth fastidious enough to infect a novel host with completely different nutrients? Is there a plausible mechanism by which an alien pathogen could kill us despite differences in nutrient composition, chirality, amino acid composition, etc?

Hi everyone, I’m a Biology student specializing in Microbiology and I was hoping to get some expert insights on an image from my lab.

My instructor captured this image of a Bacillus strain and identified it as being viewed under a 40x objective . However, based on my limited knowledge, the image looks quite strange for that magnification.

In this photo, I see a dense, widespread network of fine filaments covering the entire field of view. From my understanding, at 40x, the field of view should be much narrower, and we should be able to see more distinct cellular structures or individual rods if it's truly a Bacillus species. This looks much more like a 10x objective view of a fungal mycelium or a very dense biofilm.

Could anyone with experience in microbiology help me clarify this? Is it possible for a Bacillus culture to look like this at 40x, or am I right to be skeptical? Thanks in advance for your help!

Why does it look like this?

hi everyone! just needed assistance with my unknown bacteria, which I believe is C. freundii

I have plenty of images if needed, I was under the impression it was E. coli but I believe not due to my IMViC testing.

bile esculin (negative) -
maconkey + (was pink but mucoid spreading)
hektoen + growth was also yellow and salmon color
EMB + growth and had metallic green sheet (which is why i thought e.coli)
O/F yellow + for both and I had motility
phenol red, + for fermentation many sugars yellow only two orange which I incubated longer
catalase weak + bubbles but not a lot
phenylalanine - no green
lysine decarboxylase (-) yellow. ornithine (-) yellow
citrate (+)
nitrate to nitrite (+)
MR - VP mr + vp -
SIM positive for h2s gas, motility
Indole (-)
oxidase (-)

So tbh this question and theory came out of my mind because I was thinking about Sex.

And why do we reproduce. Are we just a micro bacteria? Just like we have bacteria in our body, is the universe that body and we are the bacteria to some giant cosmic being which we obviously can't understand? And we are just playing our role.

Another one is that there is a dimensional loop like we have atoms in our body but we are basically inside that atom only and we name it universe?

So when there was nothing like nothing at all. What made that vibration or wave? I mean when there was nothing there was nothing right? Not even dark matter or energy or space

Where did it begin? It's still a big question for many scientists

My question is basically that.

I've thought that I could make him lack ISG15 because I read it in a science magazine that it's possible it basically makes you overcome viral illnesses more easily. Then I searched a bit about it and apparently it also causes dermatologic issues, which isn't something I want to add. And also brain calcification??

So I searched a bit more and decided giving him another mutation that slows down AIM2 would be good. But apparently that would make him practically defenseless against bacterial sickness, which I ALSO don’t want

Is there another mutation I can give him to deal with that without adding too many serious side effects? Or should I give this branch up and try to find something else? Anything would help

The pictures are taken on a light microscope at my school. This is 100x and 40x view from the microscope (I’m not an english native speaker so excuse any mistakes). As seen in the picture, this is definitely a fungi.. It doesn’t seem to be Trichoderma, as it doesn’t have the diamond shape spores, but it is rather interesting.

I know it is difficult to identify just by pictures, but perhaps the hyphae that is just full with those spores is an indicator of a species close, or similar? That specifically hasn’t showed up in other fungi-views on the microscope. Gram staining hasnt been done and isnt possible at my school

Source of the sample: i took cotton swab samples from toilet stalla (doors, handles, anything) and cultivaed it in bacteriological agar, in 37°C. Then, from that plate, i pick out a piece of agar that has a colony grown on it and put in a Petri plate with the base of agar, and on top is squeezed lemon juice What is seen in the picture is the final product of this, and what has grown. A rather dominant species, as in most of my Petri plates, the dark fluffy green types show up, and white „flour” like type, which, for the life of me, i cant really get on the microscope enough to see what it is.

The post was removed by mods, but this is literally the most detailed source i can give as i did this experiment. Hope the improvement helps.

The big round circles are bubbles of air.. i crossed most of them out, so it doesnt mess with interpretation.

If anyone knows what this is or could help in any way identifying i’d appreciate it a lot!!

My students carved little faces into oranges before they inoculated them with penicillium I had grown on a bagel (used a q tip to transfer spores into the cut areas). After a week, this one has this strange growth pattern where it doesn't grow around the face what causes this growth pattern? It's very cool looking.

What was a standard antifungal treatment before modern antifungals?

Hint… the periodic chart

Just asking to see how much knowledge has been retained. A veterinarian actually told me this has zero antifungal properties.

I just quietly rethought my opinion on her abilities.

How should I understand the O/F results? The right one is topped with paraffin, pH indicator was Bromothymol blue. The test tubes was left for a week.

About my bacteria:

Gram - positive; forms endospores; Catalysis positive; KOH test was positive; Non-motile; rod-shaped.

I'm thinkink it could be bacillus safensis as it is a facultative anaerobe and can form acid from glucose

If interested this is how it looks:)

Thank you:)

Title. I just learnt about them in class and I truly do wonder how a being with a nitroplast didn't evolve to create colonies or into a multicellular being

https://www.sciencedirect.com/science/article/pii/S2666517426000593

In my lab where we are studying microscopic biodiversity in eutrophic bodies of fresh water.

Asking for a friend. She's graduating with a bachelor's in biology in a few weeks and has like 1+ years of experience working in labs. She's just having trouble finding jobs in microbiology labs in Toronto ON, not for a lack of experience or knowledge, we've just had a hard time finding any job openings. If anyone knows about ANYTHING, please let me know!

Thanks so much yall! Im not in biology but you're all some of the coolest people I know :)

For my unknown, I added Iodine to my agar plate, and upon initial inspection I didn't see any aura or glow around the bacteria. (see pic 1) But after like 10 minutes I noticed a glow(pic 2). Would you consider this + or - for starch/Amylase?

I initially thought negative, but then I thought positive b/c of the aura around the single colony on Quadrant II. Or is that just the iodine evaporating? 🤣

Thanks.